A kind of spherical virus with the size of90-140 nm was detected in some coastal shellfish by negative staining method. 报道了一种球状病毒对近海几种贝类的自然感染情况,并分别采用投喂、浸泡、划伤等方法人工感染绣凹螺、滨螺和幼鲍。
Methods Negative staining and ultrathin sections were used for electron microscopic examination. 方法负染法和超薄切片法进行检测。
Cortical microtubules were observed in intraepidermal cells of onion bulb with thin section and negative staining of protoplasts. 用超薄切片和原生质体负染的方法,在电镜下观察到洋葱鳞茎内表皮细胞周质微管的存在。
Negative staining for PR and ER may serve as a general marker of endocervical neoplasia. PR和ER的阴性表达可以作为子宫颈肿瘤的普通标记。
Results Strongly positive staining of cytokeratin-7, weakly positive staining of β-hCG in some cells, and negative staining of vimentin were observed under light and electron microscope and immunocytochemistry, suggesting they were trophoblasts. 结果①经光电镜、免疫细胞化学染色鉴定,细胞角蛋白7(CK-7)染色强阳性,β-hCG染色个别细胞弱阳性,vimentin染色阴性,表明分离培养的细胞为滋养细胞。
The effect of dimethyl sulphoxide ( DMSO) on micelle conformation of hexadecyltrimethylammonium bromide ( CTAB) was studied by means of surface tension and negative staining TEM measurements. 通过表面张力法和负染色电镜法分别从两个方面研究了二甲基亚砜(DMSO)对十六烷基三甲基溴化铵(CTAB)胶束形成的影响。
In immunocytochemistry staining, PPS and spermatogonia displayed positive staining, while Sertoli cell, round spermatid and sperm displayed negative staining. 抗c-kit抗体免疫化学染色显示:粗线期初级精母细胞、精原细胞反应呈阳性,Sertoli细胞、圆形精子细胞、精子反应呈阴性。
The results showed that the infected material could cause cytopathogenic effect ( CPE) in MDCK cells. Typical virions of CDV were observed in culture fluids with negative staining electron microscope. 结果表明:病料接种MDCK细胞产生明显的细胞病变(CPE),电镜负染观察接毒细胞培养物见有典型的犬瘟热病毒粒子。
[ Results] CD34 antigen was negative staining in neck dissection lymph node with immunohistochemistry. [结果]口腔鳞癌颈清淋巴结细胞CD34抗原免疫组化呈阴性染色。
Results: Negative staining of IL 6 was found in all the cases of normal pulpal tissue while positive in all inflammatory pulp. IL 6 expression was found mainly in monocytes macrophage and in a few vascular endothelial cells and fibroblasts. 结果:正常人牙髓组织IL-6染色为阴性,炎症牙髓组织中IL-6染色均呈阳性,主要表达细胞为单核-巨噬细胞,少量血管内皮细胞和成纤维细胞。
In order to study the change of ultrastructure of bacteria after ozone water disinfection treatment. Negative staining technique was used to carry out observation under transmission electron microscope. 氨噻肟头孢菌素对细菌超微结构的影响为研究臭氧水消毒处理后的细菌超微结构改变,采用负染色技术在透射电镜下进行了观察。
We have conducted a comparison for detecting pathogen of doubtable infectious canine hepatitis and canine viral enteritis in clinical investigation with ion catch electron microscopy and direct negative staining electron microscopy. 本研究应用离子捕获电镜技术和直接负染电镜技术比较检测了临床上疑似为犬传染性肝炎和犬细小病毒性肠炎的犬送检样品。
Only one specimen showed weak positive staining, and negative staining of NF-κ B was found in 6 cases of normal bladder tissues. 6例正常膀胱组织中仅有1例ClC-3表达弱阳性,NF-κB表达均为阴性。
The morphology and size of virion in negative staining were the same as the observation in ultrathin sections. 负染病毒粒子与超薄切片大小形态相同。
Combining Immunogold Labeling and Negative Staining Techniques to Characterize Intermediate Filament Assembled in Vitro 采用免疫胶体金标记结合负染色电镜技术对体外组装的中间纤维定性
The results by freeze etching technology, negative staining technique and transition electron microscope show that the microstructures of the two phases are completely different in ATPS. The fact that micelles and vesicles coexist in a single phase can be found too. 采用冷冻蚀刻技术、负染色技术及透射电子显微镜观察得到的结果表明,双水相中两相的微观结构明显不同,胶团和囊泡可以共存。
Results: EC images of SPI and CIN showed that there were a various of degrees of blanc epithelium stained acetic acid, abnormal blood vessel and iodine negative staining. 结果SPI和CIN的EC图像主要是不同程度的醋酸白色上皮、血管异常改变和碘阴性染色。
Results All normal bladder tissues showed negative staining for COX-2 expression, while the expression rate of COX-2 in bladder carcinoma tissues was 63.2% ( 43/ 68). 结果膀胱癌组织COX-2阳性表达率63.2%(43/68),正常膀胱黏膜组织COX-2表达均为阴性。
Under negative staining electron microscopy, the keratin was a filament with a diameter of 80-100A. 用电镜负染技术观察角蛋白细丝并测定其直径为80~100A。
Methods The phage was identified by detection of the host range, calculation of phage plaque, negative staining for TEM, serological neutralization test and one step growth experiment. 方法采用宿主范围测定、噬菌斑计数、负染色电镜观察、血清中和试验及血清交叉中和试验、一步生长实验等技术观察分离的噬菌体。
The control specimens showed negative staining. 不加第一抗体的阴性对照标本均无着色。
Normal cervical epithelium was negative staining; CIN group was weak staining in basal layer and/ or prickle cell layer; In cervical carcinoma group, the positive granulas were strongly staining around cancer nests, blood vessels and glands. 正常宫颈上皮未见明显着色;CIN组阳性表达见于基底层和棘细胞层,着色较深;宫颈癌组主要在癌巢周边表达,着色深,血管及腺体周围也有表达。
Negative staining electron microscopy ( TEM) results, the parental strains can express a complete pili, did not express mutant △ pilM. 电镜负染(TEM)结果,亲本菌株可以表达完整的菌毛,突变株△pilM菌毛缺失。
The morphologies of serum lipoproteins and subfractions were observed applying transmission electron microscopy and negative staining technique. 采用负染色透射电子显微技术观察血清脂蛋白及其亚组分的形态。
Virus particles in the extracted solution were observed under a transmission electron microscopy after negative staining with phosphotungstic acid. The results showed that there are a lot of filamentous particles about 800 nm long. 将得到的病毒提纯液经磷钨酸负染后进行电镜观察,结果表明在电镜下可看到大量线状病毒粒子的存在,大小约为800nm。